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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Methylation profiling by array of human carcinoma cells

ABSTRACT: Genomic DNA from cell lines was extracted and treated with sodium bisulfite. Fragments were PCR-amplified from the treated template DNA, labelled and hybridized to the microarray. The array contained probe sequences complementary to formerly methylated (M) and unmethylated (U) cytosines respectively. Hence the signal intensity ratio from M/(M+U) represents each cytosines methylation level in percent.

ORGANISM(S): Homo sapiens

DISEASE(S): carcinoma

SUBMITTER: Michael Boettcher

PROVIDER: E-MEXP-2698 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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