A comparative transcriptomics approach unveils networks of activity-driven neuronal gene expression

ABSTRACT: Primary cultured hippocampal neurons were infected with lentiviral particles bearing constitutively active versions of the transcription factors CREB, SRF, EGR1 and FOS to define the downstream genetic program associated with each of these paradigmatic transcription factors in neurons. For CREB, we also extracted samples at a shorter infection time to try to dissect the different components of the wider genetic response observed upon expression of a constitutively active version of this transcription factor. In order to further explore the contribution of these transcriptional regulators to activity-driven gene expression, we also treated cultured hippocampal neurons infected with GFP-only expressing lentiviral particles with different drugs (bicuculline, forskokine, and BDNF) and profiled

ORGANISM(S): Mus musculus

SUBMITTER: Eva Benito 

PROVIDER: E-MEXP-3167 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress