CREB ChIP-chip and HaloCHIP-chip experiments

ABSTRACT: Regulation of gene expression is essential for normal development and cellular growth. Transcriptional events are tightly controlled both spatially and temporally by specific DNA-protein interactions. In this study we finely map the genome-wide targets of the CREB protein across all known and predicted human promoters, and characterize the functional consequences of a subset of these binding events using high-throughput reporter assays. To measure CREB binding, we used HaloCHIP, an antibody-free alternative to the ChIP method that utilizes the HaloTag fusion protein, and also high-throughput promoter-luciferase reporter assays, which provide rapid and quantitative screening of promoters for transcriptional activation or repression in living cells. CREB ChIP-chip two biologcal replicates.

ORGANISM(S): Homo sapiens

SUBMITTER: Danette Hartzell 

PROVIDER: E-GEOD-18347 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress