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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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CREB ChIP-chip and HaloCHIP-chip experiments

ABSTRACT: Regulation of gene expression is essential for normal development and cellular growth. Transcriptional events are tightly controlled both spatially and temporally by specific DNA-protein interactions. In this study we finely map the genome-wide targets of the CREB protein across all known and predicted human promoters, and characterize the functional consequences of a subset of these binding events using high-throughput reporter assays. To measure CREB binding, we used HaloCHIP, an antibody-free alternative to the ChIP method that utilizes the HaloTag fusion protein, and also high-throughput promoter-luciferase reporter assays, which provide rapid and quantitative screening of promoters for transcriptional activation or repression in living cells. CREB ChIP-chip two biologcal replicates. HaloCHIP-chip three biological replicates with and without Forskolin

ORGANISM(S): Homo sapiens

SUBMITTER: Danette Hartzell

PROVIDER: E-GEOD-18347 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A functional analysis of the CREB signaling pathway using HaloCHIP-chip and high throughput reporter assays.

Hartzell Danette D DD Trinklein Nathan D ND Mendez Jacqui J Murphy Nancy N Aldred Shelley F SF Wood Keith K Urh Marjeta M

BMC genomics 20091027

<h4>Background</h4>Regulation of gene expression is essential for normal development and cellular growth. Transcriptional events are tightly controlled both spatially and temporally by specific DNA-protein interactions. In this study we finely map the genome-wide targets of the CREB protein across all known and predicted human promoters, and characterize the functional consequences of a subset of these binding events using high-throughput reporter assays. To measure CREB binding, we used HaloCHI ...[more]

PMID: 19860899

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Homo sapiens

Project description:CREB ChIP-chip and HaloCHIP-chip experiments

| PRJNA118157 | ENA

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