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Identification of proteins bound with RPA194 at rRNA loci in primary versus metastatic breast cancer cells

ABSTRACT: The nucleolus, the site of ribosome biogenesis, serves as a central hub for sensing and responding to cellular stress and plays a crucial role in this process; furthermore, emerging evidence highlights the potential role of ribosome biogenesis in driving metastasis. A combined approach of chromatin immunoprecipitation (ChIP) with mass spectrometry identified unique binding partners of RPA194 (the major subunit of RNA polI) in metastatic versus non-metastatic breast cancer cells.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Brandon Metge

LAB HEAD: Dr. Lalita Samant

PROVIDER: PXD060651 | Pride | 2025-07-27

REPOSITORIES: Pride

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			Action	DRS
	Mudpit_jm_20231213_Orbi1_RSamant_RPA194_10AT_1.mzid	Mzid
	Mudpit_jm_20231213_Orbi1_RSamant_RPA194_10AT_1.mzid_Mudpit_jm_20231213_Orbi1_RSamant_RPA194_10AT_1.MGF	Mzid
	Mudpit_jm_20231213_Orbi1_RSamant_RPA194_10CA_1.mzid	Mzid
	Mudpit_jm_20231213_Orbi1_RSamant_RPA194_10CA_1.mzid_Mudpit_jm_20231213_Orbi1_RSamant_RPA194_10CA_1.MGF	Mzid
	Mudpit_jm_20231213_Orbi1_RSamant_RPA194_5624_1.mzid	Mzid

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Identification of proteins bound with RPA194 at rDNA loci post radiation in breast cancer cells

Project description:Following radiation damage, cells resume ribosome biogenesis for survival. We combined chromatin immunoprecipitation (ChIP) with mass spectrometry to identify key binding partners of RPA194 (the major subunit of RNA polI) at the rDNA loci in response to irradiation.

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