Transcription profiling by array of wounded and non-wounded Anopheles gambiae mosquitoes to investigate genes regulated by wounding
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ABSTRACT: We aimed at identifying the genes regulated by wounding in Anopheles gambiae. Gene expression was compared between wounded and non-wounded mosquitoes, 3h after wounding. Wounding was induced by the injection of dsLacZ using a thin glass needle.
Project description:Elucidation of the wound response genes in Drosophila embryos Keywords: response to wounding wild-type drosophila embryos were wounded by laser ablation and compared to unwounded embryos.
Project description:Combination of both sterile wounding and infection may lead to severe health defects, revealing the importance of the balance between the intensity and resolution of the inflammatory response for the organisms fitness. Underlying mechanisms remain however elusive. Using Drosophila, we analyzed the very first steps of the process by comparing the transcriptome landscape of infected (simple hit flies, SH), wounded and infected (double hit flies, DH) and wounded (control) flies. Our objective was to identify the molecular mechanisms underlying the susceptibility of wounded flies to combined trauma and bacterial infection.
Project description:We performed RNAseq on ulcers and distant normal tissue from biopsy-wounded wild-type and Bmp4-overexpressing (Vil1-Cre;Rosa26-Bmp4) mice to investigate differences in gene expression between tissue states and genotypes. Biopsy wounding was performed by taking three biopsies from well-separated areas in the colon wall using forceps. Small tissue pieces were excised from the ulcers and distant normal colon tissue and collected for RNA sequencing.
Project description:Transcriptional profiling of laser wounded syncytial Drosophila embryos compared with control unwounded embryos. Early Drosophila embryos (Nuclear cycle 4-6) were collected, either laser wounded or not wounded, and then collected and processed immediately. Wounded and unwounded embryos express sGMCA (actin reporter)
Project description:Transcriptional profiling of laser wounded syncytial Drosophila embryos compared with control unwounded embryos. Early Drosophila embryos (Nuclear cycle 4-6) were either laser wounded or not wounded, and then collected and processed after 30 minutes. Wounded and unwounded embryos express sGMCA (actin reporter).