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Chromatin immunopreciptation genome wide location of H2A.Z binding sites in yeast yeast

ABSTRACT: genome wide location of H2A.Z binding sites in yeast Saccharomyces cerevisiae. Nucleosome density controled with H2B ChIP. Hybridization control ChIP H2AZ/H2AZ and H2A/H2B.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Luc Gaudreau

PROVIDER: E-MEXP-396 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Variant histone H2A.Z is globally localized to the promoters of inactive yeast genes and regulates nucleosome positioning.

Guillemette Benoît B Bataille Alain R AR Gévry Nicolas N Adam Maryse M Blanchette Mathieu M Robert François F Gaudreau Luc L

PLoS biology 20051101 12

H2A.Z is an evolutionary conserved histone variant involved in transcriptional regulation, antisilencing, silencing, and genome stability. The mechanism(s) by which H2A.Z regulates these various biological functions remains poorly defined, in part due to the lack of knowledge regarding its physical location along chromosomes and the bearing it has in regulating chromatin structure. Here we mapped H2A.Z across the yeast genome at an approximately 300-bp resolution, using chromatin immunoprecipita ...[more]

PMID: 16248679

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