Chromatin immunopreciptiation of H2AZ in mouse embryonic stem cells and neural precursor cells

ABSTRACT: Elucidating how chromatin organization influences gene expression patterns and ultimately cell fate is fundamental to understanding development and disease. Histone variants have emerged as key regulators of genome function by creating specialized chromatin domains. The histone variant H2AZ plays an essential, but poorly understood function during early mammalian development. Genome-wide analysis reveals that H2AZ is enriched at a large class of developmentally important genes that are known targets of Polycomb-mediated repression in embryonic stem (ES) cells. H2AZ displays a highly defined spatial patterning that is remarkably similar to the Polycomb group (PcG) protein Suz12 in ES cells, but not in differentiated cell types. By using RNA interference, we demonstrate that H2AZ is a critic

ORGANISM(S): Mus musculus

SUBMITTER: Laurie Boyer 

PROVIDER: E-TABM-556 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress