Chromatin immunopreciptiation of mouse hepatocytes to validate deep-sequencing libraries

ABSTRACT: Chromatin immunoprecipitation followed by ultra-high throughput (UHTP) sequencing (ChIP-seq) is a powerful tool to establish protein-DNA interactions genome-wide, and the primary limitation of its broad application at present is the often-limited access to sequencers. Here, we report a protocol, Mab-Seq, to generate preliminary quality evaluations and single-chromosome data for deep-sequencing libraries. We show that commercially available genomic microarrays can be used to maximize the efficiency of library creation, quickly generate preliminary data on a chromosomal scale, and help establish the depth to which novel libraries will require deep sequencing.

ORGANISM(S): Mus musculus

SUBMITTER: Dominic Schmidt 

PROVIDER: E-TABM-485 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress