A novel dominant glossy mutation causes suppression of wax biosynthesis pathway and deficiency on cuticular wax in Brassica napus

ABSTRACT: The present study reports the genetic and biochemical characterization of a dominant glossy mutant allele (BnaA. GL) in B. napus that results in a glossy phenotype. Results from transmission electron microscopy and scanning electron microscopy revealed the GL mutant exhibits reduced deposition of the cuticle layer, which was confirmed by a cuticular wax analysis. The wax compositional analysis revealed an increase in aldehydes but a severe decrease in alkanes, ketones and secondary alcohols. Genetic mapping narrowed the BnaA. GL gene to the end of A9 chromosome, where a gene homologous to ECERIFERUM1 (CER1) in Arabidopsis locates.

Then, we conducted a microarray analysis to find the differentially expressed genes between normal phenotype and glossy plants. Two comparisons were perfo

ORGANISM(S): Brassica napus

SUBMITTER: Jie Gao 

PROVIDER: E-MEXP-3989 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress