Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of Cd-induced nitric oxide synthesis in Arabidopsis

ABSTRACT: Cadmium treatment induces slow but long lasting nitric oxide production in plant tissues. This NO production can be suppressed using the commonly used Nitric Oxide Synthase inhibitor L-NAME. This inhibitor tends to partially alleviate Cd toxicity. This effect is correlated with a strong diminution of Cd content in roots of plants treated both with Cd and L-NAME compared to roots from plants treated with Cd only. The main goal of this study is the identification of transcriptionnal changes caused by Cd-induced nitric oxide, and that could potentially result in enhanced Cd root accumulation.

INSTRUMENT(S): Axon GenePix 4000A scanning hardware

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: ludivine taconnat

PROVIDER: E-MEXP-508 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Nitric oxide contributes to cadmium toxicity in Arabidopsis by promoting cadmium accumulation in roots and by up-regulating genes related to iron uptake.

Besson-Bard Angélique A Gravot Antoine A Richaud Pierre P Auroy Pascaline P Duc Céline C Gaymard Frédéric F Taconnat Ludivine L Renou Jean-Pierre JP Pugin Alain A Wendehenne David D

Plant physiology 20090123 3

Nitric oxide (NO) functions as a cell-signaling molecule in plants. In particular, a role for NO in the regulation of iron homeostasis and in the plant response to toxic metals has been proposed. Here, we investigated the synthesis and the role of NO in plants exposed to cadmium (Cd(2+)), a nonessential and toxic metal. We demonstrate that Cd(2+) induces NO synthesis in roots and leaves of Arabidopsis (Arabidopsis thaliana) seedlings. This production, which is sensitive to NO synthase inhibitors ...[more]

PMID: 19168643

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Project description:Experiment 1 Gene profiling upon biotic stress treatment Biological question : What are the genes (incuding microRNA precursors) that are differentially regulated upon flg22 treatment? Experiment description : 14-day old seedlings (Col-0 and fls2 KO) treated with 10uM of flg22 (flagellin- derived peptide) over a one hour timecourse. Experiment 2 Gene profiling in microRNA mutants Biological question : What are the genes (including miRNA precursors) that are differentially regulated in a set of microRNA mutants? Experiment description : Ler, dcl1-9, hen1-1 and hasty were grown for 12-day on MS solid medium ,seedlings were then transferred in MS liquid medium and harvested 2 days after. Experiment 3 Gene profiling in siRNA mutants Biological question : What are the genes (including miRNA precursors) that are differentially regulated in a set of siRNA mutants? Experiment description : Col-0, dcl2-1, dcl3-1 and rdr2-1 were grown for 12-day on MS solid medium ,seedlings were then transferred in MS liquid medium and harvested 2 days after. Experiment 4 Gene profiling in Agrobacterium-induced tumors and auxin-induced calli. Biological question : What are the genes (including miRNA precursors) that are differentially regulated in an Agrobacterium-induced tumors compared to auxin-induced calli? Experiment description : Col-0 plants were infected with wt Agrobacterium tumefaciens (strain A208) by stabbing the emerging stems. 18 days later, growing tumors were harvested as were the non-infected part of the stabbed-stem.In parallel, Col-0 seeds were germinated on Callus Induction Media (CIM) and 18 days later, calli were harvested. Experiment 5 Gene profiling in tasiRNA mutants Biological question : What are the genes (including miRNA precursors) that are differentially regulated in a set of tasiRNA mutants? Experiment description : Col-0, dcl4-1, rdr6-1 were grown for 12-day on MS solid medium ,seedlings were then transferred in MS liquid medium and harvested 2 days after. Experiment 6 Gene profiling upon biotic stress treatment Biological question : What are the genes (incuding microRNA precursors) that are differentially regulated upon flg22 treatment? Experiment description : 14-day old seedlings (Col-0 and fls2 KO) treated with 10uM of flg22 (flagellin- derived peptide) over a one hour timecourse.

Dataset Information

Transcription profiling of Cd-induced nitric oxide synthesis in Arabidopsis

Publications

Nitric oxide contributes to cadmium toxicity in Arabidopsis by promoting cadmium accumulation in roots and by up-regulating genes related to iron uptake.

Similar Datasets

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