Transcription profiling of Strongyloides ratti larval stage 2 (L2) direct vs indirect development

ABSTRACT: S. ratti Isofemale line ED321 Heterogonic predominantly undergoes indirect development; isofemale line ED5 Homogonic predominantly undergoes direct development. Therefore, L2 stages of ED321 Heterogonic and of ED5 Homogonic are destined for indirect (i.e. L2 indirect) and direct (i.e. L2 direct) development, respectively; these sources of material were used in this comparison. To do this, for both isofemale lines, rats were infected with ED321 Heterogonic or ED5 Homogonic and faeces collected on days 5, 6, 7 and 8 p.i. and cultured for 24 h at 19oC, after which larvae were prepared with a Baermann funnel held for 6 h at 19oC, larvae were concentrated and cleaned by sucrose flotation, as above. In excess of 75,000 worms were routinely isolated from three infected hosts. The experimental des

INSTRUMENT(S): Axon- GenePix4000B

ORGANISM(S): Strongyloides ratti

SUBMITTER: Louise Hughes 

PROVIDER: E-MEXP-709 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress