Transcription profiling of human DLD-1 cells stably expressing FOXO3a.A3-ER

ABSTRACT: In order to elucidate how FOXOs affect diverse cellular processes such as cell cycle progression, stress response and transformation we made use of an inducible version of the FOXO3a protein fused to the hormone binding domain of the human estrogen receptor (FOXO3a-A3-ER) in which all three Akt phosphorylation sites have been mutated to alanine. FOXO3a.A3-ER was stably expressed in the human colon carcinoma cell line DLD-1 (Kops et al., 2002b). In order to analyse the transcriptional response to FOXO3a activation we generated gene expression profiles from DL23 or parental DLD-1 cells, after 6 or 24 hours of 4-OHT treatment using cDNA microarrays.

INSTRUMENT(S): ScanArray 4000 [PerkinElmer]

ORGANISM(S): Homo sapiens

SUBMITTER: Almut Schulze 

PROVIDER: E-MEXP-721 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress