Transcription profiling of wild type yeast and a mutant deficient for intron degradation using a high density tiling array for genome-wide mapping of introns

ABSTRACT: High density yeast tiling array reveals new introns and extensive meiotic splicing regulation. Knowing gene structure is vital to understanding gene function, and accurate genome annotation is essential for understanding cellular function. To this end, we have developed an assay for genome-wide mapping of introns in Saccharomyces cerevisiae. Using high-density tiling arrays we compared wild type yeast to a mutant deficient for intron degradation. Our method identified 76% of the known introns, verified the existence of an additional 18 predicted introns, and revealed six new introns. Furthermore, we discovered that all 13 meiosis-specific intronic yeast genes undergo regulated splicing, which provides post-transcriptional regulation of the genes involved in yeast cell differentiation.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Molly Miranda 

PROVIDER: E-MEXP-919 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress