Transcription profiling of mouse leydig tumor cell line stimulated with either human chorionic gonadotropin alone or with bisphenol A, an endrocrine disrupting chemical

ABSTRACT: A mouse leydig tumour cell line (mLTC-1) was grown in culture and stimulated with either human chorionic gonadotropin (hCG, 10 ng/ml) alone or hCG & bisphenol A (BPA, an endrocrine disrupting chemical, 10 uM) for three hours. Cells from triplicate plates were harvested, RNA extracted, labelled and hybridised to Affymetrix Mouse genome 430 2.0 arrays. Preliminary work by RT-PCR had shown that stimulation of the cell line by BPA resulted in changes in gene expression in the spermatogenesis pathway at this time point.

ORGANISM(S): Mus musculus

SUBMITTER: unknown unknown 

PROVIDER: E-MIMR-282 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress