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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Identification of different gene expression levels in gastric cancer cell lines with drug treatment

ABSTRACT: A microarray time series was generated to identify which factor is expressed at different levels in two different gastric cancer cell lines, SNU 601 and SNU 668. These cell lines were treated for three different periods of time (24h, 48h, and 72h) with 1umol/L of olaparib or DMSO. Total RNA was extracted by using TRI reagent in accordance with the manufacturer`s instructions, and hybridized to an affymetrix GeneChip Human Gene 1.0 ST array. The results were normalized to the RObust Multi-Array Avarage (RMA) and analyzed.

ORGANISM(S): Homo sapiens

SUBMITTER: Ahrum Min

PROVIDER: E-MTAB-1012 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RAD51C-deficient cancer cells are highly sensitive to the PARP inhibitor olaparib.

Min Ahrum A Im Seock-Ah SA Yoon Young-Kwang YK Song Sang-Hyun SH Nam Hyun-Jin HJ Hur Hyung-Seok HS Kim Hwang-Phill HP Lee Kyung-Hun KH Han Sae-Won SW Oh Do-Youn DY Kim Tae-You TY O'Connor Mark J MJ Kim Woo-Ho WH Bang Yung-Jue YJ

Molecular cancer therapeutics 20130319 6

A PARP inhibitor is a rationally designed targeted therapy for cancers with impaired DNA repair abilities. RAD51C is a paralog of RAD51 that has an important role in the DNA damage response. We found that cell lines sensitive to a novel oral PARP inhibitor, olaparib, had low levels of RAD51C expression using microarray analysis, and we therefore hypothesized that low expression of RAD51C may hamper the DNA repair process, resulting in increased sensitivity to olaparib. Compared with the cells wi ...[more]

PMID: 23512992

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