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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Bulk RNA-Seq analysis of MC38 colon carcinoma cells isolated from Rag2-deficient mice

ABSTRACT: To assess the role of Bap1 genes in MC38 colon carcinoma cell linewe knocked out the Bap1 gene in the MC38 cell line through CRISPR-Cas9 technology, MC38-Bap1-Wildtype and MC38-Bap1-Knockout tumor cells expressing BFP fluorescent protein were injected into Rag2-deficient mice subcutaneously. On the 10th day of tumor growth, BFP positive tumor cells were isolated and sorted by flow Cytometer. Bulk RNA-seq was performed on MC38-Bap1-Wildtype and MC38-Bap1-Knockout tumor cells for different gene signatures.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER: Mingxia Li

PROVIDER: E-MTAB-10587 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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