Project description:In recent years,Bap1 has been reported to be involved in the process of tumorigenesis. Bap1 gene mutations frequently occur in tumors such as uveal melanoma, mesothelioma, and kidney cancer. In our study,we found that Bap1 deletion in MC38 colon carcinoma cells can promote anti-tumor immune response. To investigate how the genetic mutational landscape,whole exome sequencing of MC38 colon carcinoma cells and MC38 Bap1-knockout cells were performed.
Project description:In order to find out which genes Bap1 regulates to affect the anti-tumor immune response in MC38 colon carcinoma cell linewe knocked out the Bap1 gene in the MC38 cell line through CRISPR-Cas9 technology, RNA was directly isolated for RNA seq and were able to investigate gene expression.
Project description:Mouse-derived MC38 cells were subjected to in vivo selection to obtain colorectal cancer liver metastatic derivatives, MC38-LM3a and MC38-LM3b. Subsequently, their gene expression characteristics were identified through transcriptomic sequencing.
Project description:MC38 tumors resistant to anti-PD-1 treatment (MC38-resistant) were generated through serial in vivo passaging, and global gene expression analysis was used to compare resistant and parental tumors. MC38 and MC38-resistant tumors exhibited widespread changes in global gene expression.