MBD-Seq was applied to study the CpG methylation map of rhabdomyosarcoma cells after DiPRO1/ZNF555 inhibition

ABSTRACT: The methylation enrichment was determined by MIRA-sequencing after MBD2-capture using the MethylCollector™ Ultra Kit (Active Motif) performed on DNA samples derived from rhabdomyosarcoma cells (RMS) TE671 cells. The cells expressing shRNA targeting ZNF555 (shZNF555) were compared to the control cells expressing non-targeting shRNA (shCtl). The signals of methylation-enriched DNA (ENR) were normalised to non-enriched input DNA (INP).

INSTRUMENT(S): Qubit, Covaris S220 (LGC Genomics GmbH), Bioanalyseur 2100 (Agilent), Bravo Platform (Agilent), Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Iryna Pirozhkova 

PROVIDER: E-MTAB-10825 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress