RNAseq of Staphylococcus aureus wildtype and two-Cys-type TetR repressor GbaA deletion mutant under nonstress condition

ABSTRACT: To investigate the changes in gene expression of Staphylococcus aureus COL and the gbaA deletion mutant under nonstress condition, strains were cultivated in RPMI1640 medium (Lonza) under vigorous agitation at 37°C in triplicate until cells have reached an optical density at 500 nm of 0.5. S. aureus cells were harvested and disrupted in 3 mM EDTA/ 200 mM NaCl lysis buffer with a Precellys24 Ribolyzer. RNA isolation was performed using the phenol-chloroform-isoamylalcohol approach. After precipitation with 3 M sodium acetate and isopropanol, the total RNA was washed with cold ethanol and the pellet was solved in sterile water. Initially RNA quality was checked by Trinean Xpose (Gentbrugge,Belgium) and Agilent RNA Nano 6000 kit on Agilent 2100 Bioanalyzer (Agilent Technologies, Böblingen, Ge

INSTRUMENT(S): Illumina HiSeq 1500

ORGANISM(S): Staphylococcus aureus subsp. aureus COL

SUBMITTER: Haike Antelmann 

PROVIDER: E-MTAB-10887 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress