The global effect of hypochlorite stress on the changes in the transcriptome in Staphylococcus aureus USA300

ABSTRACT: To monitor the global changes in gene expression of Staphylococcus aureus USA300 TCH1516 under hypochlorite stress by RNA-seq, cultivation was performed in Luria Bertani (LB) medium in triplicate at 37C until cells have reached an optical density at 540nm of 2.0. Cells were harvested by centrifugation, washed with Belitsky minimal medium (BMM) and adapted to BMM for one hour before exposure to 150 M NaOCl stress. S. aureus cells of 3 replicate experiments were harvested before and 30 min after exposure to 150 M NaOCl and disrupted in 3 mM EDTA/ 200 mM NaCl lysis buffer with a Precellys24 Ribolyzer. RNA isolation was performed using the phenol-chloroform-isoamylalcohol approach. After precipitation with 3 M sodium acetate and isopropanol, the total RNA was washed with cold ethanol and the

INSTRUMENT(S): Illumina HiSeq 1500, Illumina MiSeq

ORGANISM(S): Staphylococcus aureus subsp. aureus USA300

SUBMITTER: Haike Antelmann 

PROVIDER: E-MTAB-5666 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress