Microarray analysis of Caenorhabditis elegans exposed to four indirect-acting toxicants

ABSTRACT: In this study, we exposed Caenorhabditis elegans wild types N2 to the model indirect-acting toxicants aflatoxin B1 (AFB1), benzo(a)pyrene (B(a)P), the PCB mixture Aroclor 1254 (PCB1254), and 2,3,7,8-tetrachlorodibenzodioxin (TCDD). In microarray experiments, we studied one concentration of AFB1 (30 μM), one concentration of B(a)P (30 μM), two concentrations of PCB1254 (1 μM and 30 μM), and two concentrations of TCDD (1 μM and 10 μM). As a control M9 medium with 0.5% DMSO was used. Age synchronized worms at developmental L4 larval stage were exposed to treatment for 24 hours. After flash freezing the samples, RNA was isolated, labeled and hybridized on oligo microarray (Agilent) slides.

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Inez Dinkla 

PROVIDER: E-MTAB-11143 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress