Project description:Global alterations of the early wound healing program by transcriptomics at a skin-wide level by deletion of HIF-1α in wound-infiltrating NK cells.
Project description:Changes of the transcriptional program in response to bacterial infections at a skin-wide level by deletion of HIF-1α in skin-lesions-infiltrating NK cells.
Project description:Expanded CGG repeats in the 5’UTR of fragile X messenger ribonucleoprotein 1 (FMR1) gene are translated to mutant protein containing polyglycine stretches (FMRpolyG), which evoke development of neurodegenerative disorder the fragile X-associated tremor/ataxia syndrome (FXTAS). However, little is known about the regulation of noncanonical translation of mutant FMR1 mRNA. Here, we show that insulin growth factor 2 binding proteins (IGF2BPs) directly bind to 5’UTR of FMR1 mRNA to selectively enhance biosynthesis of FMRpolyG.
Project description:FUS is one of the pathogenic RNA-binding proteins for amyotrophic lateral sclerosis (ALS). We previously reported that FUS stabilized SynGAP mRNA at its 3’UTR and maintained spine maturation and cognitive function in mice. To elucidate whether this mechanism could be pathogenic for ALS, we identified SynGAP 3’UTR variant at the binding site of FUS, different from that in mice, from a multicenter cohort in Japan. Human induced pluripotent stem cells (hiPSC)-derived motor neurons with SynGAP variant showed spine abnormality with aberrant SynGAP splicing. To evaluate how SynGAP variant altered the access of RNA binding proteins to SynGAP 3'UTR, we performed pull down assay by using biotinylated RNA probes with or without the variant.
Project description:The aim of this study was to determine the role of genes encoding polygalacturonases in strawberry fruit softening. To this purpose, several transgenic lines, cv. Chandler, were generated: plants with PG genes FaPG1 or FaPG2 downregulated, alone or in combination, by antisense transformation. Plants were grown in a confined greenhouse and fruits were harvested at the stage of full ripeness (100% of fruit surface red). The results obtained indicate that the silencing of these genes reduced fruit softening at similar level but there is not a sinergistic effect on fruit firmness.