Project description:Global alterations of the early wound healing program by transcriptomics at a skin-wide level by deletion of HIF-1α in wound-infiltrating NK cells.
Project description:Changes of the transcriptional program in response to bacterial infections at a skin-wide level by deletion of HIF-1α in skin-lesions-infiltrating NK cells.
Project description:Human primary NK cells were isolated from peripheral blood mononuclear cells (PBMCs) and cultured under two conditions: with or without cortisol (1 µM). In parallel, primary NK cells were co-cultured with K562 target cells for 6 hours in the presence or absence of cortisol (1 µM) to assess the impact of glucocorticoid exposure on NK-cell activation. Following treatment and/or co-culture, NK cells were sorted to high purity and processed for transcriptomic profiling to identify cortisol-induced transcriptional changes in resting and target-stimulated NK cells.
Project description:CEACAM5-specific CAR NK-92 cells were engineered to target CEACAM5-expressing lung tumor cells. To study their transcriptional response during tumor engagement, parental NK-92 cells, CEACAM5-CAR NK-92 cells, hydrocortisone-treated CAR NK-92 cells, NR3C1-knockout (cortisol-resistant) CAR NK-92 cells, and hydrocortisone-treated NR3C1-knockout CAR NK-92 cells were co-cultured with CEACAM5⁺ A549 lung cancer cells for 16 hours. Following co-culture, NK-92–derived effector cells were isolated by flow cytometry and processed for bulk RNA sequencing. This dataset captures transcriptional programs associated with CAR activation, hydrocortisone exposure, and glucocorticoid receptor deficiency in NK-92–based effector cells responding to CEACAM5⁺ tumor targets.
Project description:The aim of this study was to determine the role of genes encoding polygalacturonases in strawberry fruit softening. To this purpose, several transgenic lines, cv. Chandler, were generated: plants with PG genes FaPG1 or FaPG2 downregulated, alone or in combination, by antisense transformation. Plants were grown in a confined greenhouse and fruits were harvested at the stage of full ripeness (100% of fruit surface red). The results obtained indicate that the silencing of these genes reduced fruit softening at similar level but there is not a sinergistic effect on fruit firmness.