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Transcriptomic profiling of human primary NK cells treated with cortisol and/or co-cultured with K562 target cells

ABSTRACT: Human primary NK cells were isolated from peripheral blood mononuclear cells (PBMCs) and cultured under two conditions: with or without cortisol (1 µM). In parallel, primary NK cells were co-cultured with K562 target cells for 6 hours in the presence or absence of cortisol (1 µM) to assess the impact of glucocorticoid exposure on NK-cell activation. Following treatment and/or co-culture, NK cells were sorted to high purity and processed for transcriptomic profiling to identify cortisol-induced transcriptional changes in resting and target-stimulated NK cells.

INSTRUMENT(S): BD FACSAria III cell sorter, Illumina NovaSeq 6000, Novogene in-house automated library preparation system

ORGANISM(S): Homo sapiens

SUBMITTER: SOURA CHAKRABORTY

PROVIDER: E-MTAB-16365 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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