Single-Cell RNA-Seq of Memory B cells in Early Untreated Rheumatoid Arthritis Patients (Gene Expression Data)

ABSTRACT: In recent years, a circulating subset of B cells phenotypically negative for CD21 has been reported to be expanded in chronic infectious and autoimmune inflammatory diseases. While reports on the function of these cells differ by disease origin, their role in rheumatoid arthritis (RA) has remained elusive. Here, we combine clinical data with flow cytometric analyses for patients untreated for and newly diagnosed with RA. We show correlations between the frequency of CD21-Tbet+CD11c+ and destruction of both cartilage and bone in in the joints of patients at the time of diagnosis. Combining single-cell 5’ RNA sequencing with B-cell receptor (VDJ)-enriched sequencing, we are able to identify the Tbet+CD11c+ cells in the scRNA-seq data from untreated newly diagnosed RA patients. Further, we de

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Inga-Lill Mårtensson 

PROVIDER: E-MTAB-11961 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress