Transecriptome analysis of Cryptococcus neoformans exposed to ER stress inducer brefeldin A
Ontology highlight
ABSTRACT: Cryptococcus neoformans lab strain H99 was treated with 8ug/ml and 32ug/ml brefeldin A for 3h. The transcriptomes were compared to no drug treated cells.
INSTRUMENT(S): BGISEQ-500
ORGANISM(S): Cryptococcus neoformans var. grubii H99
Project description:Candida glabrata lab strain BG2 was grown in YPD broth at 37C from OD600=0.1 till 1.0. The culture was divided into three batches: no treatement (control), treated with 0.5ug/ml TUN (sub-MIC treatment) and treated with 8ug/ml tnicamycin (supra-MIC treatment). After 3 h of drug exposure, cells were harvested.
Project description:Cryptococcus neoformans lab strain H99 was spread on YPD plates supplemented with 32ug/ml and 64ug/ml iprodione. Randomly 29 (T2590-T2618( and 30 (T2619-2648) adaptors from 32ug/ml and 64ug/ml drug plate, respectively, were sequenced.
Project description:Cryptococcus neoformans lab strain H99 was exposed to 600ng/ml aureobasidin A. After 3h, transcriptome was compared to no drug treatment control.
Project description:Cryptococcus neoformans lab strain H99 was used to obtain brefeldin A adaptors. Transcriptomes of 4 adaptors, each bearing a unique aneuploid, were compared to H99.
Project description:In Candida parapsilosis, transcriptomes of two aneuploid adaptors, TJ60 and TJ74, were compared to the euploid parent #12108. Cells were grown in YPD broth at 37C from OD600=0.1 to 1.0. Cells were collected and RNA was extracted.
Project description:Candida albicans lab strain SC5314, and the aneuploid derivative TJ1031, which has segmental monosomy of chromosome R from rDNA to right telomere, were compared for the transcriptomes. Cells were grown on YPD plates for 36h. Cell pellets were collected and flash frozen in liquid nitrogen
Project description:Candida glabrata lab strain BG2 was used as parent to obtain some aneuploids by exposure to tunicamycin. Transcriptomes of 9 aneuploids bearing different chromosome change were compared to parent BG2
Project description:Two methods were used to induce petites from S, cerevisiae wild type strain MK288:.(1) MK288 was spread on YPD plate supplemented with 8 μg/ml fluconazole (2) MK288 was grown in YPD broth supplemented with 25 μg/ml ethidium bromide. Transcriptomes of the petites (TJ140 and TJ235) were compared to the wild type.
Project description:Candida albicans lab strain SC5314 was used as parent to obtain tebuconazole adaptors. Two adaptors, TJ1503 and TJ1669 were aneuploid. Transcriptomes of these two strains were compared to SC5314.
Project description:Candida auris wild type strain FY279 was used to tebuconazole adaptors. Transcriptomes of two adaptors, TJ42 and TJ44, were compared to wild type.