Exploring signal-induced cellular regulatory subnetworks by use of partial least square regression (PLSR) multidimensional analysis of gene expression time series data

ABSTRACT: By use of time series microarray experiment we identified the characteristic temporal behaviour of ~2000 new gastrin-regulated genes (accession numbers: GSE32869). To inform gastrin regulatory networks with an additional experimental dimension, we determined the temporal transcriptome in the presence of the protein synthesis inhibitor cycloheximide (CHX). For those genes where the gastrin response in the presence of CHX was higher than the response with CHX alone the gene was classified as a primary gene not dependent on de novo protein synthesis for gastrin-induced expression.Gastrin induced genes that were not upregulated in the presence of CHX were classified as secondary genes, i.e., genes whose transcriptional activation dependends on de novo protein synthesis of one or several factor

ORGANISM(S): Rattus norvegicus

SUBMITTER: Arnar Flatberg 

PROVIDER: E-MTAB-1268 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress