Transcriptome array of Pml+/+ and Pml-/- mice APL cells treated with ATRA+ATO against untreated controls

ABSTRACT: This experiment aims at understanding the main transcriptomic basals differences between Pml+/+ and Pml-/- APL blasts and during ATRA+ATO treatment. Mice were transplanted were Pml+/+ or Pml-/- blasts. Once sick, mice were treated with 12 hours of ATRA+ATO therapy (ATRA: subcutaneous diffusion, ATO: intraperitoneal injection). After treatment, bone marrow cells were harvested and blasts were sorted (GFP blasts used). After sorting, cells were frozen at -80°C (in RNA lysis buffer). Then, RNA was extracted with the Qiagen RNeasy Minikit. Samples were then hybridized on MTA 1.0 chips.

ORGANISM(S): Mus musculus

SUBMITTER: Gael Fortin 

PROVIDER: E-MTAB-13392 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress