Expression profiling of RFP-positive cells isolated from Ki67-RFP/Tcf7l2/Villin-CreERT2 mouse small intestinal epithelium 7 days after tamoxifen administration.

ABSTRACT: The transcription factor 4 (Tcf4) protein, which is encoded by the Tcf7l2 (transcription factor 7-like 2) gene, plays a key role in maintaining intestinal homeostasis, stem cell self-renewal and epithelial cell differentiation. Tcf4 has been identified as a key regulator of Paneth cell function in small intestinal crypts. It controls the expression of antimicrobial peptides and other effector molecules that contribute to the maintenance of the intestinal microbiota and protection against intestinal pathogens. Depletion of Tcf4 in mouse intestinal epithelial cells leads to loss of proliferating cells and dramatic changes in the morophology of the intestinal epithelum. To examine Tcf4 function in more detail, we used the Ki67-RFP Tcf7l2-flox/flox Villin-CreERT2 mouse strain. In this strain,

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mus musculus

SUBMITTER:  

PROVIDER: E-MTAB-13606 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): ERP155784

REPOSITORIES: biostudies-arrayexpress