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Targeted RNA-seq (TempO-Seq) of human hepatocellular carcinoma cell line HepG2, primary human hepatocytes (PHHs) and human induced pluripotent stem cell derived hepatocyte like cells (HLCs) in untreated condition

ABSTRACT: We aimed to compare the baseline expression of liver function and metabolism-related genes across the three liver models. Liver in vitro models are commonly used to study liver biology, disease mechanisms, and drug metabolism. Understanding their baseline gene expression profiles is crucial for interpreting experimental results and selecting the most appropriate model for specific research questions. Experimental workflow Cell Culture: Culture HepG2, PHHs, and hiPSCs-derived HLCs under standard, untreated conditions. Cell lysis: Collect RNA lysates using BioSpyder 2x enhanced lysis buffer from each cell type. Sequencing: Ship lysates to BioClavis, 201 Dumbarton Rd, Glasgow G81 4XJ, United Kingdom and sequence using TempoSeq targeted sequencing technology: https://www.bioclavis.co.uk/temposeq Data Analysis: Perform quality control of the sequencing results, normalize the data and compare normalized gene expression profiles, focusing on liver function and metabolic genes across the three models.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Homo sapiens

SUBMITTER: Tamara Danilyuk

PROVIDER: E-MTAB-15401 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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