Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of engineered K562 cell line with t(7;12)(q36;p13) treated with SNX-2112 and OSI930

ABSTRACT: K562-t(7;12) cells (K562 background with CRISPR/Cas9 engineered t(7;12) translocation) were treated with SNX-2112 (210 nM), OSI930 (9.19 µM), or DMSO (control) (0.1%) for 24 hours.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Denise Ragusa

PROVIDER: E-MTAB-15526 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

RNA-seq of Kit+CD24+ fractions in K562-t(7;12) compared to K562-control

Project description:K562 cells with a CRISPR-engineered t(7;12)(q36;p13) translocation (K562-t(7;12)) and control line transiently transfected with Cas9 only (K562-ctrl) were FACS sorted on positivity to surface markers Kit and CD24.

2025-09-12 | E-MTAB-15530 | biostudies-arrayexpress

RNA-seq of engineered K562 cell line by CRISPR/Cas9 generation of t(7;12)(q36;p13) chromosomal translocation

Project description:We recreated the t(7;12) translocation in K562 cells by CRISPR/Cas9 to understand its effects on haematopoietic cells, which is of relevance to understand how this cytogenetic abnormalities causes and promotes acute leukaemia in infants. Wild-type K562 were edited by electroporation of ribonucleoprotein complexes consisting of Cas9 enzyme and two guide RNAs targeting patient-specific breakpoint loci. K562 electroporated with Cas9 enzyme only were used as control. Edited K562 harbouring the t(7;12) were single-cell cloned to obtain homogeneous populations (hereby referred to as K562-t(7;12)). We performed RNA sequencing analysis of K562-t(7;12) compared to K562 control to uncover transcriptional changes associated with the translocation.

2022-09-06 | E-MTAB-11851 | biostudies-arrayexpress

RNA-seq of MCF10A transduced with an inducible lentiviral MYB expression system

Project description:The non-tumourigenic breast cell line MCF10A was transduced using pINDUCER21-MYB vector to express MYB upon addition of doxyclyclin (DOX), and compared to an empty vector (EV) control (pINDUCER21 (ORF-EG)) with and without the addition of DOX

2023-12-01 | E-MTAB-12977 | biostudies-arrayexpress

RNA-seq of adenoid cystic carcinoma (ACC) ACCX11 cells compared to normal salivary glands

Project description:PDX-derived ACCX11 adenoid cystic carcinoma cells were compared to normal salivary gland (NSG) controls.

2023-12-01 | E-MTAB-12978 | biostudies-arrayexpress

ChIP-seq of MYB in adenoid cystic carcinoma (ACC) ACCX11 cells

Project description:Chromatin immunoprecipitation was carried out using an anti-MYB antibody in PDX-derived ACCX11 adenoid cystic carcinoma cells. Input samples were extracted prior to the addition of antibody.

2023-12-01 | E-MTAB-12976 | biostudies-arrayexpress

Transcriptomic profiling of human primary NK cells treated with cortisol and/or co-cultured with K562 target cells

Project description:Human primary NK cells were isolated from peripheral blood mononuclear cells (PBMCs) and cultured under two conditions: with or without cortisol (1 µM). In parallel, primary NK cells were co-cultured with K562 target cells for 6 hours in the presence or absence of cortisol (1 µM) to assess the impact of glucocorticoid exposure on NK-cell activation. Following treatment and/or co-culture, NK cells were sorted to high purity and processed for transcriptomic profiling to identify cortisol-induced transcriptional changes in resting and target-stimulated NK cells.

2025-12-18 | E-MTAB-16365 | biostudies-arrayexpress

Transcriptome changes in leaves of the DEFENSE NO DEATH 1 (DND1) silenced potato plants

Project description:Silencing of DND1 in potato leads to resistance to late blight, powdery mildew and Botrytis cinerea. At the same time, however, it reduces plant growth and causes leaf necrosis. To get knowledge on the molecular events behind the pleiotropic effect of DND1 downregulation in potato transcriptome analysis were performed on three DND1 silenced lines in comparison with the potato cultivar ‘Désirée’ as a wild-type.

2024-10-22 | E-MTAB-13824 | biostudies-arrayexpress

RNA-seq of MOLM-13 acute myeloid leukemia cell line treated with single and combination 5-Azacytidine and CB-5083

Project description:MOLM-13 acute myeloid leukemia cells were treated with 7 µM 5-Azacytidine (Cayman Chemical 11164), or 450 nM CB-5083 (Cayman Chemical 19311), or in combination, or 0.1% DMSO as control. Treatments were conducted for 48 hours.

2023-04-22 | E-MTAB-12848 | biostudies-arrayexpress

RNA sequencing of mouse embryonic stem cells (mES) and hemogenic gastruloids with MNX1 overexpression

Project description:Bulk RNA sequencing of 3-dimensional gastruloid differentiation system initiated from mouse embryonic stem (ES) cells and biased towards hemato-endothelial cell production with overexpression of MNX1 by lentiviral vector transduction

2022-10-10 | E-MTAB-12173 | biostudies-arrayexpress

RNA-seq of Cryptomonas sp. under phototrophic and glucose supplemented phototrophic conditions, together with 3 dissolved organic carbon concentrations

Project description:Cryptomonas sp. was grown under phototrophic conditions, glucose supplemented phototrophic conditions and 3 different dissolved organic carbon (DOC) concentrations: 1.5, 30 and 90 mg C l−1. The objective was to study the adaptations that make Cryptomonas sp. thrive under high DOC conditions.

2022-07-12 | E-MTAB-11370 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data