AtCRK1 positively regulates salt stress tolerance in Arabidopsis through the modulation of stress-responsive genes

ABSTRACT: Plant molecular improvement group at University of Shizuoka, Japan, previously identified 18 potential salt tolerance mutants using an activation-tagging approach at the cellular level. In one of these mutants, stc8, two genes AtbHLH106 and AtCRK1 were activated adjacent to the borders of T-DNA. AtbHLH106 is known to regulate salt stress by interacting with the G-box (CACGTG) present in the promoters of abiotic stress responsive genes. Here we report that AtCRK1 contributes to salt-stress regulation in Arabidopsis. In this study, TAIL-PCR revealed that AtCRK1 was present 5567 bp from the RB of the T-DNA and activated on both MS without salt and MS supplemented with 150 mM NaCl. Real-time PCR showed that AtCRK1 was enhanced 1.7 times on MS without salt and 2.7 times on MS with 150 mM NaCl.

INSTRUMENT(S): Affymetrix GeneChip

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Aftab Ahmad 

PROVIDER: E-MTAB-15761 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress