Project description:We wanted to explore the function of MG53 in THP-1 cells, so we treated THP-1 cells with control b-gal or IRF7 adenovirus, and using PMA to induce THP-1 differentiated into macrophages. Thus exploring how MG53 affect macrophages.
Project description:We wanted to explore the function of MG53 in THP-1 cells, so we treated THP-1 cells with control b-gal or MG53 adenovirus, and using PMA to induce THP-1 differentiated into macrophages. Thus exploring how MG53 affect macrophages.
Project description:To identify differentially expressed genes between apatinib-sensitive and apatinib-resistant HUVECs, one apatinib-sensitive and one -resistant primary human umbilical vein endothelial cells (HUVECs) were treated with apatinib for 48 hours. After treatment, cells were harvested and total RNA was extracted. The quantity and integrity of the RNA were evaluated and sequencing libraries were constructed.
Project description:We compared transcriptome of monocyte-derived macrophages of 5 patients with GBA-PD (4 L444P/N, 1 N370S/N) and 4 asymptomatic GBA mutation carriers (GBA-carriers) (3 L444P/N, 1 N370S/N) and 4 controls. We also conducted comparative transcriptome analysis for L444P/N only GBA-PD patients and GBA-carriers. Revealed deregulated genes in GBA-PD independently of GBA mutations (L444P or N370S) were involved in immune response, neuronal function. We found upregulated pathway associated with zinc metabolism in L444P/N GBA-PD patients. The potential important role of DUSP1 in the pathogenesis of GBA-PD was suggested.
Project description:TEAD1 emerged as an estrogen-sensitive gene that was upregulated in a HFD mouse model and in NAFLD patients. We investigated the roles of TEAD in NAFLD by treating primary human hepatocyte spheroids with two small molecule inhibitors blocking the TEAD/YAP interaction. One small molecule blocks the interaction by inhibiting TEAD autopalmitoylation (TEADap), the second small molecule blocks the interaction by binding to the surface of the TEAD protein (TEADsf).
Project description:The aim of this study was to investigate the role of strigolactones (SLs) in controlling tillering and their involvement as a signal for nitrogen status. RNA-seq analysis was performed on 18-day-old hydroponically grown Tad17 triple knock-out TILLING mutant plants (SL-deficient mutant) and WT-segregant plants, 8 days after introducing them to nitrogen limiting conditions. The experiment consisted of two different treatments: High Nitrogen (10 mM N) and Low Nitrogen (0.1 mM N). Each treatment included six biological replicates. Total RNA was extracted from pooled samples of four basal node sections per biological replicate. In this study, the basal node was defined as the 0.5 cm section from the base of the main shoot, comprising the apical meristem, lateral buds, leaf meristems, etc.
Project description:Metabolites produced by human gut microbiome have a profound influence on brain health with increasing associations to Parkinson’s disease pathology that lack a mechanistic insight. Using Caenorhabditis elegans model expressing human α-synuclein, we systematically tested key microbial fermentation products and identified succinate as a potent driver of pathology. As succinate administration was found to alter major PD associated pathological end-points, we further investigated the changes at transcriptional level by performing the whole worm transcriptome profiling of the wild-type strain. Through differentially expressed genes (DEGs), we examined the extent of physiological impact exerted by an exogenously administered metabolite and tried to comprehend the mechanism through which succinate generates a proteotoxic environment that promotes aggregation of alpha-synuclein in a transgenic C. elegans strain expressing human alpha-synuclein. It also helped us to identify the molecular pathways that result in mitochondrial dysfunction and substantiate our findings.
Project description:To investigate the transcriptional changes induced by the fer mutant in tomato roots, roots from 3-week-old seedlings of fer, cultivated in 1/2 Hoagland’s nutrient solution, along with their respective wild-type control groups, were harvested. RNA-seq analysis was conducted with three biological replicates using the TruSeq RNA Sample Prep Kit.
Project description:Four L. sativus accessions, exhibiting varying levels of resistance to E. pisi (resistant, partially resistant, partially susceptible, and susceptible), were analysed using dual RNA-seq. Leaflets from each plant were sampled at 0 (non-inoculated), 6, 12, 24, 48, and 72 hours after inoculation. Forty six samples were sequenced by lllumina Novaseq PE150 at STABvida sequencing provider (Lisbon, Portugal).