Single-cell RNA sequencing analysis of left atrial appendage tissue following magnetogel-based occlusion in a porcine model
Ontology highlight
ABSTRACT: This study aims to evaluate the long-term biocompatibility and tissue integration of a novel magnetogel-based left atrial appendage (LAA) occluder. To assess the host tissue response, single-cell RNA sequencing (scRNA-seq) was performed on LAA tissue samples obtained from a preclinical porcine model after 10 months of magnetogel implantation. Tissue samples were collected from regions in direct contact with the magnetogel (contact zone) and adjacent non-contact areas. scRNA-seq analysis revealed the cellular composition, which included myofibroblasts, fibroblasts, neutrophil cells, macrophages, T cells, mast cells, smooth muscle cells, and Schwann cells. Critically, no significant differences in the ratios of immune cells (e.g., neutrophils, macrophages, T cells) or mast cells (indicative of allergic reactions) were observed between the contact and non-contact zones.
INSTRUMENT(S): Sterile surgical instrument set (scalpels, tissue forceps, dissection scissors), animal operating table, biological safety cabinet, 10x Genomics Chromium Controller, PCR thermocycler, Agilent 2100 Bioanalyzer with High Sensitivity DNA chip (for quality control), Illumina NovaSeq 6000, Water bath (for enzymatic dissociation), cell strainer (70-μm), benchtop centrifuge, automated cell counter/hemocytometer, biological safety cabinet
ORGANISM(S): Sus scrofa
SUBMITTER: zeng linjun
PROVIDER: E-MTAB-15847 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
ACCESS DATA