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Genome-wide CRISPR knockout library screening coupled with high-throughput sequencing to analyze DNA repair outcomes

ABSTRACT: Cells were transduced with a genome-wide CRISPR knockout library to introduce gene disruptions across the genome. After Cas9-induced DNA double-strand breaks, repair outcomes were captured and quantified using high-throughput sequencing. This experiment aims to identify genetic factors influencing specific DNA repair outcome patterns such as 1-bp insertions, microhomology-mediated deletions, and large insertions.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Yanbin Wan

PROVIDER: E-MTAB-15874 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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