RNA-seq of human fibroblasts during pseudo-longitudinal in-vitro aging and long-term Metformin or Rapamycin treatment

ABSTRACT: This dataset contains longitudinal transcriptomic profiles of human fibroblasts across in-vitro aging and pharmacological modulation with Metformin and Rapamycin. Longitudinal sampling of human cells is inherently challenging. Thus, we model aging in non-proliferative fibroblast cultures maintained at confluency, which recapitulate key transcriptomic signatures of in-vivo aging. Four female donor fibroblast lines were cultured for six months in a non-proliferative state, with sampling every 30 days to capture pseudo-longitudinal transcriptomic changes. Parallel cultures were treated with the anti-aging compounds Metformin or Rapamycin over the same period to assess their impact on age-associated transcriptional trajectories. We compare transcriptomic aging changes of in-vitro aging to a co

INSTRUMENT(S): Illumina NovaSeq X

ORGANISM(S): Homo sapiens

SUBMITTER: Larissa Traxler 

PROVIDER: E-MTAB-16089 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress