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Single-cell transcriptional profiling of healthy brain, primary and post-irradiation recurrent murine glioblastomas using 10x Genomics Flex chemistry

ABSTRACT: This study characterizes the single-cell transcriptional landscape of murine glioblastoma generated using the RCAS/Nestin-Tv-a system, in which hPDGFB and shTP53 transgenes drive tumor initiation. We profiled primary and post-irradiation recurrent tumors, along with healthy brain controls, using the 10x Genomics Flex (GEM-X) gene expression platform and custom probe addon sets targeting the RCAS transcripts. A total of 16 samples were included after QC filtering (primary tumors, recurrent tumors, and control brains). The data set provides genome-wide single-cell RNA-seq measurements at probe-level resolution.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER: David Lindgren

PROVIDER: E-MTAB-16532 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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