RNA-seq analysis of human hepatocyte Huh7 cells transfected with CRISPR/(d)Cas9 plasmids for transcriptional activation of APOA1 and PON1

ABSTRACT: The aim of the present study was to obtain and characterize an in vitro model for endogenous APOA1 and PON1 long-time up-regulation in hepatocytes that can be further used to decipher the mechanism of their protective action. Cultured human hepatocytes (HuH-7 cell line) were transfected with CRISPR/dCas9 activation plasmids targeting APOA1/PON1 genes. Following selection with specific antibiotics, bulk RNA sequencing was used for the transcriptomic characterization of the transfected hepatocytes. Hepatocytes from human hepatocarcinoma (Huh7 line, Cell Lines Service GmbH, Germany) were cultured in RPMI-1640 supplemented with FBS (10%, v/v), penicillin (100U/mL), and streptomycin (0.1mg/mL). Huh7 cells were seeded into 12 well plates at a density of 70.000 cells/well in complete RPMI media,

INSTRUMENT(S): HISAT2, TOPHAT2, Illumina NovaSeq X

ORGANISM(S): Homo sapiens

SUBMITTER: Loredan Stefan Niculescu 

PROVIDER: E-MTAB-17152 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress