Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

7 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MiRNA transcription profiling by array of PINK1 mutant mice

ABSTRACT: Analysis of micro RNA expression in the brains (cortex) of 6 months-old PINK1 knockout mice.

INSTRUMENT(S): Agilent Microarray scanner (G2505C)

ORGANISM(S): Mus musculus

SUBMITTER: L. Miguel Martins

PROVIDER: E-MTAB-2462 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

The identification of putative circulating miRNA biomarkers of Thioacetamide-induced hepatotoxicity.

Project description:The Thioacetamide-treated rat was first identified as a model of hepatotoxicity by Gupta in 1956 and is now well-established, not least because the histopathogical output closely mimics that seen in humans with chronic liver disease. Acute treatment of rats with Thioacetamide causes pronounced necrosis and inflammation. Animals received intraperitoneal (ip) doses of vehicle-only (0.9% (v/v) saline) (n=3), or 100 mg/kg Thioacetamide (n=3) and were sacrificed after 24 hours. Blood was withdrawn via the descending vena cava and immediately transferred into potassium/EDTA tubes. Following centrifugation (16,100g, 4M-BM-0C, 5 min) the plasma was collected and stored at -80M-BM-0C. miRNA microarray profiling of RNA extracted from the plasma of rats treated with Thioacetamide revealed that a subset of miRNAs were differentially expressed following treatment. These miRNAs appeared to mediate pathways involved in hepatic fibrosis and stellate cell activation, suggesting that they might function as predictive biomarkers following compound-induced hepatotoxicity. The changes correlated well with increases in ALT levels, which are the current gold standard method for determining the extent of liver injury. Furthermore, it is hypothesised that particular aetiologies of liver damage might cause differing expression profiles of miRNAs, thus certain miRNAs could be implemented in a panel-type expression study to distinguish between different types of hepatic injury. Single channel miRNA microarrays were performed on n= 3 samples, 2 treatment groups; control and test. Control animals received vehicle-only (0.9% (v/v) saline) via the ip route. Test animals received 100 mg/kg Thioacetamide dissolved in 0.9% (v/v) saline, via the ip route. 24 h after dosing animals were sacrificed using decapitation under terminal anaesthesia.

2014-03-14 | E-GEOD-55885 | biostudies-arrayexpress

mRNA transcription profiling by array of PINK1 mutant mice

Project description:Analysis of mRNA expression in the brains (cortex) of 6 months-old PINK1 knockout mice.

2014-07-20 | E-MTAB-2461 | biostudies-arrayexpress

Transcription profiling of rac1 knock-down zebrafish embryos

Project description:Phenotypic, proteome and transcriptome analysis of rac1 knockdown in zebrafish embryos

2008-12-31 | E-MEXP-1593 | biostudies-arrayexpress

Transcription profiling of wild type dorsal root ganglia (DRG) taken from mice 10-12 weeks, DRG taken from NT-4 -/- mice of 4-5 weeks, NT-4 -/- mice of 12 weeks

Project description:Gene expression profiling was carried out to compare labeled cRNA derived from 3 experimental groups. Group 1 was mRNA extracted from wild type dorsal root ganglia (DRG) taken from mice 10-12 weeks of age, Group 2 was mRNA extracted from DRG taken from NT-4 -/- mice of 4-5 weeks of age. Group 3 was mRNA extracted from DRGs taken from NT-4 -/- mice of 12 weeks of age. In all cases mRNA was extracted from 6-10 mice per group. The experiment was carried out a total of three times.

2012-04-19 | E-MEXP-10 | biostudies-arrayexpress

Transcription profiling of mouse primary neural stem cells (neurospheres) originating from separate isolations (individuals), different passages and parallel cultures

Project description:Investigation of transcriptome variability in neurospheres originating from separate isolations (individuals), different passages and parallel cultures. As a control neurospheres were also compared to neurospheres induced to differentiate by adding serum and plating onto solid support.

2005-03-31 | E-MEXP-297 | biostudies-arrayexpress

Transcription profiling of mouse adult primary neural stem cells when stimulated with the proliferative agent PACAP (pituitary adenylate cyclase-activating polypeptide)

Project description:Investigation of transcriptional changes induced in adult primary neural stem cells when stimulated with the proliferative agent PACAP (pituitary adenylate cyclase-activating polypeptide). In addition the data was compared to results obtained from a proliferation control (stimulation with a transmembrane receptor agonist) and a differentiation control (primary neural stem cells induced to differentiate by withdrawing growth factors, adding serum and plating onto solid support).

2005-05-15 | E-MEXP-322 | biostudies-arrayexpress

Transcriptomic comparison of chlorhexidine tolerant and sensitive Salmonella Typhimurium

Project description:Transcriptional profiles of a chlorhexidine tolerant Salmonella Typhimurium were compared to its, chlorhexidine sensitive, isogenic progenitor isolate. RNA was extracted from mid-log phase cells from both isolates, without chlorhexidine exposure and following exposure to 1 µg/ ml of chlorhexidine for 30 minutes. Transcriptional profiles of the tolerant isolate were compared to the sensitive isolate, with and without chlorhexidine exposure. Carried out using 3 biological replicates for each sample; each sample hybridised in a two-channel hybridization against Salmonella genomic DNA as the comparator/reference

2015-06-06 | E-GEOD-59566 | biostudies-arrayexpress

Transcription profiling of Candida albicans clinical isolates grown under identical growth conditions in NGY medium

Project description:Comparison of clinical isolates grown under identical growth conditions

2009-02-11 | E-MEXP-1931 | biostudies-arrayexpress

Transcription profiling of indole-3-acetic acid at physiological concentrations (IAA) treatment of Arabidopsis seedlings

Project description:To demonstrate the analysis of differential expression using CATMA v1 arrays, Arabidopsis seedlings were treated with indole-3-acetic acid at physiological concentrations. The seedlings were germinated in liquid medium, and treated with indole-3-acetic acid for 30, 120 or 240 minutes. Changes in expression patterns were monitored using a complete loop design including an untreated sample (0 minutes). Reciprocal labelling was used rendering a total of eight hybridisations. An additional self-to-self hybridisation for time-point 0 was included. The statistical analysis was based an ANOVA model and indicated that 1123 GSTs were differentially expressed (p < 2.37 x 10-6) in at least one of the three time points following treatment.

2004-05-31 | E-MEXP-65 | biostudies-arrayexpress

Transcription profiling of 10-day old Arabidopsis seedlings treated for 30, 120 and 240 minutes with the plant hormone indole-3-acetic acid (auxin)Auxin induced effects investigated using bead-purified probe arrays to assess the technology

Project description:21120 Arabidopsis thaliana gene sequence tags were amplified and purified using a novel bead-based strategy (see Publication for more details).This experiment was carried out to demonstrate the use of these arrays for expression profiling of Arabidopsis thaliana.Briefly, 10-day old Arabidopsis seedlings were treated for 30, 120 and 240 minutes with the plant hormone indole-3-acetic acid (auxin) and the effects on gene expression were analysed.

2004-12-31 | E-MEXP-140 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data