Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

70 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

AngioEvasion_001

ABSTRACT: We aim to identify the molecular mechanisms underlying LLC tumor evasion from endogenous angiogenesis inhibitor Endostatin in-vivo. Tumor cells were inoculated in C57/BL6 mice. Mice were treated with muFc-endostatin (20ﾵg/day) until the tumors evade therapy. Tumors were re-implanted in a new batch of mice for 4 consecutive in-vivo passages. Passage four (P4) endostatin-treated and control tumors were profiled. Significantly upregulated genes attributed to endostatin resistance were identified. Their gene expression levels were assessed by real-time PCR (Taqmanﾮ probe). Candidate genes were further functionally evaluated in-vivo using inhibitors.

ORGANISM(S): Mus musculus

SUBMITTER: Amir Abdollahi

PROVIDER: E-MTAB-2529 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of human dermal fibroblasts and lung microvascular endothelial cells irradiated with iron ions

Project description:Normal human dermal fibroblasts (NHDF) and human lung microvascular endothelial cells (HMVEC-L) were irradiated with iron ions (0, 0.2, 0.4 and 1 Gy, 1GEv/n) at Brookhaven National Labs (BNL). Aim of the study is to find differentially transcribed genes in dependance of radiation dose/source and cell type.

2009-04-25 | E-TABM-374 | biostudies-arrayexpress

Transcription profiling of wild type, MKK3 transfected and Ras transfected mouse fibroblasts which have invaded or not invaded through a separating membrane

Project description:Invasiveness of genetically modified cells is tested. Four cells lines (NIH3T3 untreated control; NIH-Ras positive control which is invasion +; NIH-MKK3actK4 and NIH-MKK3actATN, two constructs with MKK3 gene which shall be investigated for theit invasiveness). Cells on top as well as cells from bottom of separating membrane (those which had "invaded") form all 4 cell lines are collected and expression profiled. Aim is to find genes which are correlated to "invasion".

2009-09-18 | E-TABM-561 | biostudies-arrayexpress

Transcription profiling of human dermal fibroblasts and lung microvascular endothelial cells irradiated with protons

Project description:Normal human dermal fibroblasts (NHDF) and human lung microvascular endothelial cells (HMVEC-L) were irradiated with protons (0, 0.5, 1 and 2 Gy, 1GEv/n) at Brookhaven National Labs (BNL). Aim of the study is to find differentially transcribed genes in dependence of radiation dose/source and cell type.

2009-04-25 | E-TABM-379 | biostudies-arrayexpress

Transcription profiling by array of human colorectal cancer cell lines expressing NVM-1

Project description:The aim of this experiment was to determine the molecular function of NVM-1 Gene in tumor invasion and metastasis.

2011-08-16 | E-TABM-1061 | biostudies-arrayexpress

Transcription profiling of prostate tumor cell line AT1 inocculated into rats and then subjected to carbon ion or photon radiation

Project description:In the present study approximately 1 to 2 mm3 prostate tumor AT1 was inoculated subcutaneously in the right hind leg of adult male Copenhagen rats. When the tumor diameter exceeded 15 mm, tumors of 5 and 4 rats were irradiated with carbon ion radiation of 37 or 16Gy respectively. Tumors of 5 other rats were irradiated with photon radiation of 37Gy. One animal irradiated with 37 Gy carbon ion radiation and one animal irradiated with photon radiation was sacrificed 12h, 30h, 72h, 7d and 14d after irradiation respectively. One animal irradiated with 16 Gy photon radiation was sacrificed 12h, 60h, 7d and 14d after irradiation respectively. Non-irradiated animals were sacrificed at 60h time point. Tumors were dissected and frozen in liquid nitrogen immediately. Total RNA from tumor material was isolated using the NucleoSpin RNA L kit (#740962.20, Macherey-Nagel). Differential gene expression analysis was performed on the Agilent whole rat genome Oligo Microarray (44k) platform by comparative two dye hybridisation with dye-swaps.

2008-07-18 | E-TABM-481 | biostudies-arrayexpress

Transcription profiling of mouse lung fibroblasts and Lewis lung cells to investigate the transciptional regulation that governs cell-cell fusion process

Project description:This study aimed to investigate the transciptional regulation that governs cell-cell fusion process.

2008-06-30 | E-TABM-381 | biostudies-arrayexpress

Transcription profiling of human blood from patients with acute kidney injury following open-heart surgery with cardiopulmonary bypass

Project description:Acute kidney Injury (AKI) following open-heart surgery with cardiopulmonary bypass (CPB) is associated with an increased morbidity and mortality. The goal of this study is to explore patterns of gene expression in human AKI following CPB. This prospective observational study was approved by the Institutional Review Board Human Subjects Committee. Design and methods: Peripheral blood RNA samples were collected prospectively from a cohort of 30 patients at Caritas St. Elizabeths Medical Center in Boston, MA, undergoing CPB at time points immediately prior, and two and 24 hours following CPB. Gene expression patterns of four patients who developed AKI (cases) following CPB will be compared with six patients matched for clinical characteristics, who did not develop AKI (controls). AKI was defined by an incremental increase in serum creatinine of 30% within 72 hours following CPB. For peripheral blood RNA sampling, 2.5 ml of venous whole blood was collected into PAXgene Blood RNA Tube collection tubes (PreAnalytiX GmbH, Hombrechtikon, CH). Whole blood RNA was extracted with the PAXgene Blood RNA extraction Kit according to the manufacturers instructions (PAXgene RNA Kit Handbook, 06/2005). All biological samples are stored at 80° C.

2009-12-12 | E-TABM-595 | biostudies-arrayexpress

Transcription profiling of human breast cancer and scarcoma tissue and adjacent tissue to investigate alterantive splicing

Project description:investigation of alternative splicing in breast tumors and sarcomas

2017-07-05 | E-MEXP-1670 | biostudies-arrayexpress

Transcription profiling by array of rat ASNEOpancreas tumor cell line transplanted into a rat and the primary tumor and lymph node metastases profiled

Project description:Target of this study is to identify genes which are responsible for lymph node metastasis or primary pancreatic tumors. 1ASNEO is a pancreas tumor cell line, derived from a tumor which does not (hardly) metastasize. 1ASNEO cells are implanted into rat to induce tumor growth in transplanted rat. Cells from the induced primary tumor as well as from lymph node metastases are excised cultivated and profiled. Aliquots of these tumor cell lines are implanted in a subsequent rounds into other animals, reisolated and also profiled

2011-08-18 | E-TABM-1059 | biostudies-arrayexpress

Transcription profiling of human A549 lung cells after infection with influenza virus with and without virus protein PB1-F2 knocked out

Project description:Effect of the influenza virus protein PB1-F2 on the host innate immune response.

2010-02-12 | E-MEXP-2090 | biostudies-arrayexpress