Project description:To identify genes hypermethylated and transcriptionally downregulated, we have employed the Illumina Infinium HumanMethylation450 BeadChip and Agilent SurePrint G3 Human Gene Expression 8x60K. And we validated selected genes in cases and controls. eventually we estalished CIMP of MDS.
Project description:MLLENL transformed primary cells were treated for 6h with 250nM flavopiridol, PC585 or DMSO as control. RNA was isolated and analyzed on Agilent SurePrint G3 Mouse GE 8x60K (Agilent Microarray Design ID 028005) arrays.
Project description:SurePrint G3 Human Gene Expression 8x60k oligonucleotide microarrays (Design ID: 039494,Agilent) were used to characterize gene expression profiles of HepG2/C3A cells exposed to HBCD (hexabromocyclododecane) or DMBA (dimethylbenzanthracene) for 24 hours.
Project description:Expression Profiles of SUM149 cell treated by ACY1215 at 0h, 3h, 6h and 12h by Agilent SurePrint G3 Human GE v3 8x60K. Expression Profiles of SUM149 cell treated by ACY1215 at 0h, 3h, 6h and 12h
Project description:We performed expression profiling using microarray technology. The expression profiling identified genes or mechanism potentially regulating the proliferation of human lung cancer cells by BoxA of HMGB1 and Alu-siRNA transfection. The RNAs of our experiment were hybridized with Agilent SurePrint G3 Human GE 8X60K, V3 Microarrays (Agilent®).
Project description:Pax5, a transcription factor, is expressed exclusively in pro B cells. To understand the genes regulated by Pax5 in epithelial cells, we overexpressed Pax5 in lung cancer cell line A549 and examined the change of transcription profile using Agilent SurePrint G3 Human Gene Expression 8x60K v2 Microarray (Agilent Technologies).
Project description:SurePrint G3 Human Gene Expression 8x60k oligonucleotide microarrays (Design ID: 028004,Agilent) were used to characterize gene expression profiles of A549 cells exposed to different concentrations of HBCD (hexabromocyclododecane) for 24 hours.