Wheat endosperm development

ABSTRACT: Our objective is to describe variations in expression profiles of transcriptional factors during endosperm development, especially during filling phase in which storage proteins are synthesized. Plants from cv. Recital were cultivated under controled conditions. Two biological repetitions were available for grain harvest. at 11 developmental stages (132, 172, 289, 323, 381, 437, 477, 527, 577, 635 and 686 degrees C day). RNA was extracted from all samples of seeds whose embryo was removed.cDNA synthesis, labelling, and hybridization were made according to manufacturer’s protocol (NimbleGen Arrays Users Guide). The SuperScript Double-Stranded cDNA synthesis kit (invitrogen) was used to transform RNA in double-stranded cDNA. The first strand was transcribed with an oligo dT primer and th

ORGANISM(S): Triticum aestivum

SUBMITTER: Catherine Ravel 

PROVIDER: E-MTAB-2952 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress