Transcriptome profiling of Sinorhizobium meliloti Rm1021 grown in semisolid media (MM) in presence of 2-tridecanone (2-TDC)
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ABSTRACT: This experiment captures the genes differentially expressed of the Sinorhizobium meliloti reference strain (Rm1021) when this bacteria is grown in semisolid media (MM 0.6%) in presence of a compound which promotes surface motiliy
Project description:With this transcriptome we want to know differentially expressed genes in a double mutant GR4fadD525 (affected in fadD gene and smc00525, gene of unknown function) compared to GR4fadD under more permissive conditions for surface spreading (MM 0.6% Noble agar).
Project description:Hfq-dependent transcriptional alterations in the nitrogen-fixing endosymbiont S. meliloti. Comparison: S. meliloti 1021 strain Vs S. meliloti 1021Dhfq (containing a deletion of the hfq ORF).
Project description:RctR encodes a protein belonging to the GntR family of transcriptional regulators and is involved in regulation of conjugative transfer of symbiotic plasmids of Sinorhizobium meliloti. In order to identify genes differentially expressed in a 1021RctR mutant, the transcriptome of S. meliloti 1021RctR was compared with that of the wild-type 1021, using Sm6kOligo DNA microarrays. Cells of 1021 and 1021RctR were grown at 30C in TY broth to mid logarothmic phase (OD600nm)=0.7-0.8 before RNA isolation.
Project description:Transcriptome profiling by array of the Sinorhizobium meliloti GR4-derivative mutant GNS577, affected in ntrY and ntrX genes, to identify possible cell processes regulated by this system
Project description:S. meliloti genes differently expressed in the wildtype 1021, the phosphatidylethanolamine-deficient mutant CS111, or the phosphatidylcholine-deficient mutant OG10017 were identified by determining and comparing the whole genome transcription profiles of these three strains. Mutants and wildtype present different phenotypes and the molecular bases for these differences was explored by transcriptome analysis.
Project description:S. meliloti 1021FDC5 was grown at 30ºC in 20 ml of TY broth with shaking to late logarithmic phase (optical density at 600 nm = 1-1.2). After incubation, cells were pelleted, washed twice in MM and resuspended in 2 ml of the latter medium. For time course experiments in liquid, 0.5 ml of the inoculation culture was added to 50 ml of fresh MM. At various times, samples were removed for determining viable cells counts as well as for RNA isolation/preparation (7 and 14 hours). For experiments on plates, 20 ml of MM containing 0.7% (Semisolid) or 1.3% (Solid) agar was dispensed onto each Petri dish and allowed to gel. The plates were air dried at room temperature for 15 min. 0.1 ml of the inoculation culture was plated onto the surface of the plates and allowed to dry for 10 min. The plates were then inverted and incubated at 30ºC.
Project description:Transcriptional responses occurring in the spleen in response to N. caninum infection compared to uninfected mice Two conditions: infected and un-infected mice; Biological replicates: three (or four) infected and three uninfected mice. One control and one infected per array