Intestinal epithelial HuR balances counteracting RNA networks to protect against intestinal inflammation and cancer: PART1 Translation profiling

ABSTRACT: Elavl1/HuR is a ubiquitous and conserved RNA-binding protein that binds to a U-rich RNA motif that shuttles between nucleus and cytoplasm. In epithelia, the elevated expression of HuR assumingly promotes degeneration and cancer suggesting that its generic suppression may provide clinical benefits. In this study we focused on biological and clinical functions of HuR in intestinal epithelial cells and we presented evidence that changes in HuR levels induce polarized distortions in these cells to support different pathologic outcomes. For translation profiling cytoplasmic fractions of CMT93 sublines, containing monosomes and polysomes were separated as in (Katsanou et al., 2009). Fractions containing monosomes and polysomes were collected using a retriever 500 fraction collector (Teledyne Is

INSTRUMENT(S): Scann Array Express

ORGANISM(S): Mus musculus

SUBMITTER: Dimitris Kontoyiannis 

PROVIDER: E-MTAB-4016 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress