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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Arabidopsis thaliana strain:Columbia-0Transcriptome or Gene expression

ABSTRACT: To gain initial insight into the regulatory mechanisms by which signalling in response to an elevated CO2 concentration exerts CA1- and CA4-dependent repression of stomatal development, we conducted high-throughput RNA-seq transcriptomics on immature aerial tissues of A. thaliana seedlings grown at the low (150 p.p.m) and elevated CO2 concentrations (500 p.p.m). Hypocotyls and cotyledons of developing seedlings (5 DAG; WT and ca1 ca4 mutant plants; n>1,000 per sample) grown in the low and elevated CO2 concentrations were used as source tissue to extract total RNA and conduct RNA-seq experiments using the HiSeq 2000 platform (Illumina). The raw data from three independent biological replicates (experiments).

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Julian Schroeder

PROVIDER: E-MTAB-4447 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Carbonic anhydrases, EPF2 and a novel protease mediate CO2 control of stomatal development.

Engineer Cawas B CB Ghassemian Majid M Anderson Jeffrey C JC Peck Scott C SC Hu Honghong H Schroeder Julian I JI

Nature 20140706 7517

Environmental stimuli, including elevated carbon dioxide levels, regulate stomatal development; however, the key mechanisms mediating the perception and relay of the CO2 signal to the stomatal development machinery remain elusive. To adapt CO2 intake to water loss, plants regulate the development of stomatal gas exchange pores in the aerial epidermis. A diverse range of plant species show a decrease in stomatal density in response to the continuing rise in atmospheric CO2 (ref. 4). To date, one ...[more]

PMID: 25043023

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