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The effects of naltrexone on gene expression in HCT116 colorectal cancer cell line

ABSTRACT: A number of reports have identified a putative anticancer role for naltrexone when used at doses lower than those conventionally administered. In particular, it has been shown that these low doses of naltrexone are able to suppress tumour growth. A definitive mechanism of action has yet to be established, but what is known is that the effect can be via modifications to the host immune system rendering it more anticancer in nature, or through direct antagonism of tumour growth. Our current paper uses gene expression as a way of unraveling this.The human colorectal cancer cell line HCT116 was cultured in medium alone, or with naltrexone at two different doses for 4 hours. Cells were either harvested after this time or transferred into fresh medium for a further 24 hours before being harvested. RNA was isolated from these cells by Trizol. Total RNA was labelled and hybridised to Illumina Human HT-12v4 arrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Jayne Dennis

PROVIDER: E-MTAB-4454 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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