Transcription profiling of 3 genotypes in mouse

ABSTRACT: For transcription profiling six mouse T cells samples were analyzed: 3 genotypes 2 samples for each genotype were complared. Two wt animals-control;2 animals containing deficiency allele (Df11(1)) on chromosome 11 and 2 with the reciprocal duplication on chromosome 11(Dp11(1)). The part of the chromosome 11 deleted or duplicated is 0.8Mb between Gast and Hcd17b genes. Splenic CD4+ T cells were purified using anti-CD4 MiniMacs beads (Miltenyi Biotech, Bergisch Gladbach, Germany) according to the manufacturer's protocol and were plated at 106 cells/ml in complete RPMI 1640 medium (Sigma) supplemented with 10% fetal bovine serum (FBS), 0.3 mg/ml L-glutamine, 10 micromolar 2-mercaptoethanol, 100 U/ml penicillin, and 0.1 mg/ml streptomycin in 6 well plates coated overnight with anti-CD3 (5 _g/m

ORGANISM(S): Mus.Musculus

SUBMITTER: Olga Ermakova 

PROVIDER: E-MTAB-447 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress