Kinetics of host transcription in CEFs productively infected with recombinant fowlpox virus vaccine vector FP9

ABSTRACT: Fowlpox virus (here as FP9, a plaque-purified, high passage-attenuated derivative) effectively suppresses induction of the ‘innate’ immune responses, notably the Type I interferon system, of the permissive host (chicken). Despite the extensive usage of fowlpox virus as a recombinant vaccine vector in chickens, its immunomodulatory mechanisms remain largely unknown. In this study, a transcriptomic analysis using the Affymetrix GeneChip chicken genome array was performed at the host gene transcription level at 4, 8, 16 and 24 hours post-infection of mock treated and FP9-infected (MOI=5, 2h) chicken embryo fibroblasts (CEF). The experiment was performed in triplicate with three different batches of CEFs. Because fowlpox virus is capable of expressing antigens in mammalian cells, these st

ORGANISM(S): Gallus gallus

SUBMITTER: Michael Skinner 

PROVIDER: E-MTAB-5455 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress