ABSTRACT: mRNA from tumor cells, tumor associated macrophages and tumor associated T cells from high grade serous ovarian carcinoma patients was sequenced.
Project description:Transcriptome of high grade serous ovarian carcinoma isolated from patient acites and associated macrophages was analyzed using RNASeq.
Project description:Ascites-associated macrophages were collected from human ovarian carcinoma patients and their expression profiles determined via Agilent microarrays. In addition, monocyte derived macrophages from healthy donors were differentiated ex-vivo with MCSF and GMCSF and their expression profiles determined as well.
Project description:The transcriptome of serous ovarian cancer tumor associated macrophages was characterized. Additionally, their transcription response to PPARD agonists and inverse agonists in cell culture was analyzed.
Project description:Primary cells from high grade serous ovarian carcinoma patients, derived from ascites and omentum were sequenced to study signaling networks.
Project description:This study presents a cell-type–resolved quantitative proteomics analysis of paired primary and recurrent ascites samples from patients with high-grade serous ovarian carcinoma (HGSOC). Ascites samples were collected from three patients (IROC60, IROC65, and IROC126) at diagnosis and upon disease recurrence following platinum–taxane chemotherapy. For each sample, ascites-derived cells were fractionated into unsorted, CD45⁻ (tumor-enriched), and CD45⁺ (immune-enriched) populations using magnetic-activated cell sorting. Using label-free LC–MS/MS proteomics, we quantified proteome-wide changes associated with disease recurrence across distinct cellular compartments. Pathway-level analyses revealed consistent suppression of interferon signaling across tumor and immune fractions, alongside enhanced oxidative phosphorylation and metabolic reprogramming in recurrent tumor cells. Immune-enriched fractions exhibited signatures of immunosuppression, including reduced antigen presentation and enrichment of regulatory and monocyte-related pathways. Together, this dataset provides a comprehensive proteomic resource for investigating tumor–immune interactions and recurrence-associated molecular remodeling within the ovarian cancer ascites microenvironment.
Project description:Chemotherapies have been shown to enhance anti-tumor immunity, but whether chemotherapies affect tumor-associated macrophages (TAMs) is still unclear. We found TAMs is different before and after chemotherapy by comparing proteomic profiles of TAMs before and after chemotherapy.
Project description:The secretomes of tumor cells, and macrophages from high grade serous ovarian carcinoma from the ascites of patients undergoing primary surgery were analysed after 24h culture (ex vivo) via obitrap.
Project description:CAFs, ascTAMs and M1- and TAM-like-MDMs were treated with 1 μM of the Prostacyclin analoga MRE and DMSO (as solvent control). The aim of this experiment is to see which effect MRE (Prostacyclinanaloga) has on RNA expression (activation of signaling pathways). Background: Prostacyclin receptor expression is elevated in TAMs. Prostacyclinsynthase expression was detected mainly in CAFs. (