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Fibroblasts potentiate melanoma cells in vitro invasivity induced by UV-irradiated keratinocytes - transcription profiling

ABSTRACT: Melanoma represents a malignant disease with steadily increasing incidence. Despite a remarkable enrichment of therapeutic repertoire achieved in the last decade, primarily limited sensitivity to therapy or acquired resistance are common. These phenomena limit survival of patients in advanced stages of the disease. UV-irradiation is a very important factor in melanoma initiation. In this study, we tested the influence of normal dermal fibroblasts as well as cancer-associated fibroblasts isolated from melanoma on UV-irradiated keratinocytes as an inductor of melanoma cell migration in 3-D collagen gels. The introduction of normal dermal fibroblasts and mainly cancer-associated fibroblasts to such system further significantly stimulated melanoma cells invasivity. A panel of candidate gene products responsible for facilitation of melanoma cells invasion was defined with emphasis on IL6, IL8 and CXCL1.

ORGANISM(S): Homo sapiens

SUBMITTER: Karel Smetana

PROVIDER: E-MTAB-5973 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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